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FeaturedAmpliSeq™ Custom Solutions: From Gene List to Results You can now sequence hundreds of genes of your choice from just 10ng of DNA. Create custom ultrahigh-multiplex PCR panels with Ion AmpliSeq™ Designer, a new free online assay design tool. The custom Ion AmpliSeq Panels contain up to 1,536 amplicons per tube which require only 10 ng of input DNA for target selection. Get started today and go from DNA to variants in a single day.
Join our live discussion and see for yourself how Ion AmpliSeq™ Custom Solutions can transform targeted DNA sequencing in your lab.
Live event! Flow Cytometry Educational Series Register for this event or other previously recorded Flow University webinars Cell Health Educational Series How to VideosCheck in often to view the latest video tutorials. Each video takes you through the basics in under 5 minutes. Click on the how-to video of interest: |
Training Webinars
Courses available for Real-Time PCR, Next Generation Sequencing, Semiconductor Sequencing, Capillary Electrophoresis Sequencing and many others. |
Select your area of interest to view available seminars
Sequencing
Semiconductor Sequencing
Ion Torrent technology uses a massively parallel array of proprietary semiconductor sensors to perform direct real-time measurement of the hydrogen ions produced during DNA replication. A high-density array of wells on the ion semiconductor chips provides millions of individual reactors while integrated fluidics allow reagents to flow over the sensor array. This unique combination of fluidics, micromachining, and semiconductor technology enables the direct translation of genetic information (DNA) to digital information (DNA sequence), rapidly generating large quantities of high quality data.
View the latest recorded Ion Torrent webinars
- Semiconductor sequencing training webinars
- Introduction to Semiconductor Sequencing
- Introduction to Ion Torrent Informatics
- Technical Performance of Ion Torrent Semiconductor Sequencing
Next Generation Sequencing
The SOLiD™ System is a highly accurate, massively parallel next-generation sequencing platform that supports a wide range of applications. The flexibility of independent run lanes in the microfluidic FlowChip and multiplexing capability allow you to run a single lane quickly or conduct multiple experiments in a single run. With scalable throughput and up to 99.99% accuracy, the SOLiD™ System enables you to complete your sequencing and tag experiments more cost effectively than previously possible.
- SOLiD™ training webinars
- Demonstration of the SOLiD™ EZ BeadTM system
- Discovering Personalized Tumor Biomarkers
- Whole Cancer Genome Sequencing: Progress Toward common Application
- Fundamentals of 2 Base Encoding and Color space
Capillary Electrophoresis
We set a new standard in capillary electrophoresis with the 8-capillary 3500 and 24-capillary 3500xL Genetic Analyzers. The 3500 8-capillary platform can run a wide variety of applications —including de novo sequencing and resequencing (mutational profiling)—as well as microsatellite analysis, MLPA™, LOH, MLST, AFLP® and SNP validation or screening.
uTYPE® 6.0, a rapid and easy-to-use SBT data analysis software
Introducing uTYPE® 6.0, a rapid and easy to use data analysis software for HLA Sequence Based Typing. Our technical expert will demonstrate data interpretation from a single user interface, navigate easily through key positions, eliminate mismatched allele combinations from consideration, and customize reports containing interpreted results for convenient review and submission.
Presenter: Keith Kurutz, HLA Field Application Specialist, Life Technologies
60 min recorded webinarRecorded July 20, 2011
SeCore® HLA Sequence-Based Typing-The NIH Experience
In this webinar, Sharon Adams, MT, CHS (ABHI) outlines NIH Bone Marrow program and its HLA typing workflow, presents real life use case and technical differences between Life Technologies SeCore® and other SBT vendors, discusses points to consider while setting up SBT and discusses experience working with Life Technologies
Cloning
Gateway® recombination cloning technology circumvents traditional restriction enzyme based cloning limitations, enabling you to access virtually any expression system. Widely adopted in the research community with more than 1,500 references since its launch, Gateway® technology makes collaboration across research disciplines easy and convenient and enables access to a multitude of vectors from these research groups for truly multidisciplinary scientific studies.
- MultiSite Gateway Cloning - 1hr online seminar
- Gateway Cloning - 1hr online seminar
- Advanced cloning - Gateway and Vector NTI - 1hr online seminar
MembranePro™ Protein Expression System
For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably.
Biotherapeutics
Monoclonal Antibody Expression-Multi-milligram to Gram Scale Protein Production in Mammalian Cells in 5 Days
Understand the key elements of scalable transient protein production in CHO and HEK293 cells Learn the ins and outs of producing milligrams to grams of proteins such as monoclonal antibodies
Cell-Based Assays- Advanced Platforms for Discovery and Validation of Protein Therapeutics
Discover and validate your therapeutic antibody with greater speed and precision Quantify therapeutic protein functionality using cellular assays that monitor ligand–receptor interactions in an HTS-validated format
Protein Microarrays- High-Content Protein Microarrays for Biomarker Discovery and Therapeutic Response Monitoring
Learn how functional protein microarrays can empower your biomarker identification efforts Find out how other clinical researchers are advancing their research with protein microarrays
Cell Culture
Myths and Mysteries of Cell Culture
Original Air Date: Wednesday November 16, 2011
A complimentary seminar discussion brought to you by Gibco® cell culture featuring Timothy Fawcett, Ph.D. Director, BioTechnical Institute of Maryland.
We discuss Cell Culture Best Practices & Tips for Improving Your Culture Outcomes
- Understanding the in-vitro system and factors affecting it
- Working with components of cell culture media & formulations, serum performance characteristics
- Cell culture productivity and interrelationship between cell growth cycle & its environment
- Tips & techniques for maximizing recovery
Gibco® cell culture products are infused with quality, customer-focused innovation, and service excellence from beginning to end. From the most basic formulations to the newest innovations, Gibco® products always provide the highest quality, consistency, and performance—for results you can count on every day.
Cell Culture Basics Video Series
Cell Culture Basics is a series of five introductory videos to cell culture, covering topics such as laboratory setup, safety, and aseptic technique. You'll also find basic methods for passaging, freezing, and thawing cultured cells.
View any of the five video's
| This video provides an overview of the basic equipment used in cell culture and proper laboratory set-up. | This video is focused on the steps you should take to prevent contamination of your cell culture. | Video 3 in the five part series explains why, when and how to passage cells grown in both adherent and suspension cultures. | |||
| This video demonstrates the critical steps required to freeze cells while maintaining optimal cell health. | Video 5 presents the best way to thaw cells without harming them in this stressful process. |
Stem Cell Advancement
Single Cell analysis: unlocking variability in single cells
A one-hour on-demand webinar recorded live July 21, 2011 covering Single cell profiling of stem cells and identification of subpopulations
Learn how to easily perform qPCR from single stem cells
Listen to this on-demand 45 min webinar recorded live on July 6, 2011
Single-Cell Profiling of Stem Cells and Identification of Subpopulations, a free webinar introducing our new Single Cell-to-CT™ kit that can be used to easily and repeatedly perform qPCR from single cells.
Presented by: Richard Fekete, an Ambion® R&D team leader
Looking for What Is New in Mesenchymal Stem Cell Culture?
This seminar will review mesenchymal stem cell (MSC) research and how better-defined_ cGMP manufactured and regulatory-friendly reagents have become a necessity to translate MSC research from bench to bedside. Join scientists from GIBCO® to discuss new products and techniques in a 1 hour live web seminar you can attend right from your desk.
Stem Cell Systems Seminar Featuring ES/iPSC‘s Similarities, Differences, and Technologies to Detect Them
One-hour pre-recorded webinar.
Proteins, Expression, Isolation & Analysis
1hr online seminar featuring ‘Neon Transfection System: Overcoming the hurdles of efficient DNA and siRNA transfection into primary cells, stem cells and other difficult to transfect cells’
Drug Discovery
Cryopreserved HepaRG™ Cells: An Alternative In Vitro Screening Tool for Drug-Drug Interaction and Safety Applications
During this 60 min webinar recorded live July 2011, we demonstrate the utility and reproducibility of differentiated, cryopreserved HepaRG™ Cells in hepatic screening applications. We show data which demonstrate that cryopreserved HepaRG™ Cells are metabolically competent and maintain functional xenobiotic sensing pathways similar to those exhibited in PHH cultures. Finally, we show the utility of cryopreserved HepaRG™ Cells in the evaluation of intrinsic and metabolically-activated toxicity.
Presented by Dr. Jonathan P. Jackson, Research Scientist, Life Technologies
Applications of a kinase binding assay
Two part seminar series highlighting the versitility of the LanthaScreen Eu Kinase Binding Assay to help your assay development challenges.
Physiologically Relevant Tools and Personalized Support for Your Drug Discovery Research -Original Air Date: Tuesday, October 18, 2011
In this webinar, Mark Federici, Strategic Alliances Manager for Drug Discovery, will highlight how Life Technologies can support your research with relevant tools and services, collaborative partnerships, and customizable solutions
Bioproduction
View recordings
- Learn from the experts about how the Applied Biosystems® POROS®XS resin is ideal for process-scale bioseparations. This breakthrough resin combines optimal particle size, pore size and surface chemistry to provide superior process performance.
- Cell Culture Contaminant Detection: The Power of Rapid Molecular Technology to Detect Mycoplasma, Vesivirus, and Mouse Minute Virus in Under 4 Hours on 8/18/2010
- Perform Accurate CHO Host Cell DNA Quantitation In-house in under 5 Hours
- CHO Host Cell DNA Quantitation In-house in under 5 Hours on 6/29/2010
Real-time PCR
View list of Gene Expression Analysis Training Webinars
View list of Genotyping and Genetic Variation Training Webinars
Keeping pace with Next Generation Sequencing: Next Generation qPCR
In this complimentary on-demand webinar, we will introduce the main applications of Next Generation real time qPCR and how it is beginning to revolutionize molecular genetics. Learn how the evolution of qPCR has brought powerful analyses tools such as single-cell gene analysis and Digital PCR, while also improving workflow and flexibility.
Platinum® Multiplex PCR Master Mix.
Recorded live: 1/18/2012
A detailed look at multiplex PCR, a powerful tool for genotyping, gene expression analysis and other applications. In addition, introduce one of the newest members of the Life Technologies family of products, the Platinum® Multiplex PCR Master Mix, a new highly specific optimization-free formulation for multiplexing PCR.
Robust cDNA synthesis with SuperScript® III Reverse Transcriptase.
Recorded live: 1/18/2012
A critical component of gene expression research is achieving robust cDNA synthesis. Suboptimal reverse transcriptase reactions can lead to low yields of cDNA or truncated cDNA. Learn why SuperScript® III reverse transcriptase, which has been engineered for higher thermostability, can provide higher yields of full length cDNA for complete gene expression.
TaqMan® Protein Assays: Protein Detection and Quantification using Proximity Ligation and Real-Time PCR
TaqMan® Protein Assays is a new technology that enables quick and easy identification and relative quantification of proteins from limited quantities of cell and tissue lysates. It is an adapted form of PLA™, a proximity ligation assay technology that combines antibody–protein binding with real-time PCR-based detection of the reporter nucleic acid sequence. These assays have successfully been used for a range of small sample applications—from monitoring cellular differentiation (loss of pluripotency), to monitoring gene silencing, to screening for protein biomarkers and protein:protein interactions. Whether studying microRNA target analysis, or screening cells for low expression proteins, TaqMan® Protein Assay products provide a novel technology, enabling the highest sensitivity for quantitative protein analysis.
Cells-to-CT™ Kits: Expression analysis without RNA purification
With Cells-to-CT™ Kits, you can perform expression analysis directly from cultured cells without RNA purification, and without a loss in performance.
Protein assays on real-time PCR? Watch and learn.
TaqMan® Protein Assays: Protein Detection and Quantification Using Proximity Ligation and Real-Time PCR.
Speaker: Elana Swartzman PhD, Senior Application Scientist, Life Technologies
Imaging & Flow
An Introduction to Immunofluorescent Staining of Cultured Cells
Recorded October 5th, this webinar discusses the steps of an immunofluorescent staining protocol including material list, common variations, and necessary controls. Also covered: simple troubleshooting guide, examine how to avoid common pitfalls, Q&A from the live event
A Comparison of Basic Immunofluorescent Labeling Strategies
In this complimentary webinar, we will compare different immunofluorescent labeling strategies exploring the pros and cons of each method. You will learn when the use of a direct conjugate is appropriate and when amplification techniques can be utilized. We'll also present a simple decision tree to aid in determining the best method for each situation.
An Introduction to flow cytometric analysis using Molecular Probes® reagents- A 2 Part Series
In this complimentary 2-part series, we will give an overview of tools and techniques using Molecular Probes® reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis.
Part 1
An Introduction to Flow Cytometric Analysis Using Molecular Probes® Reagents Part 1: Cell proliferation analysis
Original Air Date: Monday, November 21, 2011Part 2
Viability, vitality, and apoptosis analysis
Recorded live on: Thursday, January 12, 2012
In this webinar we discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring Membrane structure, Mitochondrial function, Metabolism, Caspase activity, Membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes.
