J774 mouse macrophage cell.
A J774 mouse macrophage cell sequentially stained with BODIPY FL ganglioside GM1 (B13950) and then with Alexa Fluor 555 dye–labeled cholera toxin subunit B (Cat. No. C22843, C34776; also available as a component of Cat. No. V34404). The cell was then treated with an anti–CT-B antibody (a component of Cat. No. V34404) to induce crosslinking. Alexa Fluor 555 dye fluorescence (top panel, red) and BODIPY FL dye fluorescence (middle panel, green) were imaged separately and overlaid to emphasize the coincident staining (bottom panel, yellow). Nuclei were stained with blue-fluorescent Hoechst 33342 (Cat. No. H1399, H3570, H21492).