Bovine pulmonary artery endothelial cells (BPAEC). LysoTracker® Red DND-99 and MitoTracker® Green FM.
Bovine pulmonary artery endothelial cells (BPAEC) incubated simultaneously with 50 nM LysoTracker® Red DND-99 (Cat. no. L7528) and 75 nM MitoTracker® Green FM (M7514) at 37°C for 30 minutes. Both dyes showed excellent cellular retention, even after cells were fixed in 3% glutaraldehyde for 30 minutes. The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/).