Mouse fibroblasts. Paclitaxel, biotin-XX goat anti–mouse IgG antibody and ELF® 97 Cytological Labeling Kit
Mouse fibroblast microtubules labeled with a mouse monoclonal anti–ß-tubulin antibody in conjunction with biotin-XX goat anti–mouse IgG antibody (Cat. no. B2763) and then developed for visualization with alkaline phosphatase–mediated techniques using the ELF® 97 Cytological Labeling Kit (Cat. no. E6603). This kit's novel ELF® 97 phosphatase substrate yields a yellow-green–fluorescent precipitate at the site of alkaline phosphatase activity. Prior to antibody labeling, mouse fibroblasts were fixed and permeabilized in the presence of cytoskeletal stabilizing buffer and treated with paclitaxel (Cat. no. P3456) to stabilize microtubule structures. The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/).