Live HeLa cells incubated with Alexa Fluor® 594 transferrin to label early endosomes.
Live HeLa cells incubated with Alexa Fluor® 594 transferrin (Cat. no. T13343) for 10 minutes to label early endosomes. The cells were subsequently fixed with paraformaldehyde and labeled with an antibody to the endosomal protein RhoD. That antibody was visualized with a green-fluorescent secondary antibody. Yellow fluorescence indicates regions of co-localization. To illustrate the staining pattern, the cells were imaged by both fluorescence (left panel) and differential interference contrast (DIC) microscopy (right panel). The image was contributed by Harry Mellor, University of Bristol.