Cryostat section of mouse kidney. FluoCells® prepared slide #3, Alexa Fluor® 488 wheat germ agglutinin, Alexa Fluor® 568 phalloidin and DAPI.
FluoCells® prepared slide #3 (Cat. no. F24630) containing a 16 µm cryostat section of mouse kidney stained with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261), red-orange–fluorescent Alexa Fluor® 568 phalloidin (Cat. no. A12380) and blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490). The image represents an optical section obtained by simultaneous two-photon excitation of all three dyes at 797 nm using a Bio-Rad Radiance 2100 multi-photon microscope system. The image was acquired at the 2001 3-D Microscopy of Living Cells Course, University of British Columbia, Vancouver, Canada, by John Jordan, Bio-Rad Laboratories; and Iain Johnson, Life Technologies.