Zebrafish larva reticulospinal neurons. Alexa Fluor® 488 tyramide.
Horseradish peroxidase (HRP)–filled reticulospinal neurons of the hindbrain of a whole-mount zebrafish larva were detected using green-fluorescent Alexa Fluor® 488 tyramide (TSA Kit #2, Cat. no. T20912). The spinal cord was labeled by transection in the presence of HRP (1 mg/mL). After a two-hour incubation, the specimen was fixed and the HRP was visualized using Alexa Fluor® 488 tyramide. This figure represents a projection (performed with AutoQuant Imaging, Inc., software) of a stack of 30 images.