Mouse intestine. Alexa Fluor 488 goat anti–chicken IgG conjugate, Alexa Fluor 568 phalloidin and DAPI.
A section of mouse intestine stained with a combination of fluorescent stains. Fibronectin, an extracellular matrix adhesion molecule, was labeled using a chicken primary antibody against fibronectin and visualized using green-fluorescent Alexa Fluor 488 goat anti–chicken IgG antibody (A11039). The filamentous actin (F-actin) prevalent in the brush border was stained with red-fluorescent Alexa Fluor 568 phalloidin (A12380). Finally, the nuclei were stained with DAPI (D1306, D3571, D21490).