Immunohistochemical detection using tyramide signal amplification.
Immunohistochemical detection using tyramide signal amplification. A transverse section of fixed zebrafish retina was probed with mouse monoclonal FRet 34 antibody and subsequently developed for visualization using HRP-conjugated goat anti–mouse IgG antibody and Alexa Fluor® 488 tyramide, which are supplied in the TSA Kit #2 (Cat. no. T20912). The section was counterstained with the blue-fluorescent Alexa Fluor® 350 wheat germ agglutinin (Cat. no. W11263) and the far red–fluorescent TOTO®-3 nuclear stain (Cat. no. T3604).