NIH 3T3 cells. Anti-GFP Alexa Fluor® 594 conjugate and DAPI.
NIH 3T3 cells that were transiently transfected with a Green Fluorescent Protein (GFP) expression vector, then plated and allowed to attach and proliferate. The cells were fixed and labeled with our Alexa Fluor® 594 conjugate of the anti-GFP antibody (Cat. no. A21312). Cells expressing GFP show dual labeling of both GFP (green fluorescence) and the anti-GFP antibody (red fluorescence). In this overlay of fluorescence and differential interference contrast (DIC) micrographs, the GFP-transfected cells exhibit green and red signals that overlap to yield yellow, and DAPI (Cat. no. D1306, D3571, D21490) stains the nuclei with a light-blue fluorescence. In the cells that are not transfected, the DAPI-stained nuclei exhibit a bright blue fluorescence.