Fluorescent probes generated with ARES DNA Labeling Kits hybridized to human metaphase chromosome spreads. Centromere probes specific for chromosomes 17, 1 and 15 were prepared by nick translation and labeled with kits containing green-fluorescent Alexa Fluor 488 (A21665), red-orange–fluorescent Alexa Fluor 546 (A21667) and red-fluorescent Alexa Fluor 594 (A21669) dyes. DNA was counterstained with the blue-fluorescent Hoechst 33342 dye (H1399, H3570, H21491), and the slides were mounted using the ProLong Antifade Kit (P7481). This multiple-exposure image was obtained with bandpass filter sets appropriate for fluorescein, rhodamine, Texas Red dye and DAPI.