Bovine pulmonary artery endothelial cells.
The subcellular structures in fixed and permeabilized bovine pulmonary artery endothelial cells are visualized with several fluorescent dyes. Filamentous actin (F-actin) was identified with Alexa Fluor 633 phalloidin (Cat. No. A22284), which is pseudocolored magenta. Lipophilic regions of the cell, including intracellular membranes, were stained with green-fluorescent DiOC6(3) (Cat. No. D273). Finally, nuclei were counterstained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). The image was acquired using filters appropriate for fluorescein and DAPI and a special filter (courtesy of Omega Optical) for the Alexa Fluor 633 dye, consisting of a narrow band exciter (630DF10), dichroic (640DRLP) and emitter (660DF10).