Multinucleate HeLa cell in metaphase. DAPI, anti–bovine a-tubulin, mouse monoclonal 236-10501, biotin-XX conjugate and Alexa Fluor® 568 streptavidin.
A multinucleate HeLa cell in metaphase that was fixed and then stained with a combination of fluorescent dyes. The chromosomes were stained with DAPI (Cat. no. D1306, D3571, D21490). The cytoskeleton was detected with the biotin-XX conjugate of mouse monoclonal anti–a-tubulin antibody (Cat. no. A21371), which was then visualized with red-fluorescent Alexa Fluor® 568 streptavidin (Cat. no. S11226). The multiple-exposure image was acquired using filter sets appropriate for rhodamine and DAPI.