Bacillus subtilis sporulation.
Correlated fluorescence imaging of membrane migration, protein translocation, and chromosome localization during Bacillus subtilis sporulation. Membranes were stained with red-fluorescent FM® 4-64 (Cat. No. T3166, T13320). Chromosomes were localized with the blue-fluorescent nuclear counterstain, DAPI (Cat. No. D1306, D3571, D21490). The small, green-fluorescent patches (top row) indicate the localization of a GFP fusion to SpoIIIE, a protein essential for both initial membrane fusion and forespore engulfment. Progression of the engulfment is shown from left to right. Green fluorescence in the middle and bottom rows demonstrates fully engulfed sporangia stained with MitoTracker® Green FM (Cat. No. M7514). Full details of the experimental methods and interpretation are published in Proc Natl Acad Sci U S A 96, 14553 (1999). Image contributed by Kit Pogliano and Marc Sharp, University of California at San Diego. Reproduced from the 7 December 1999 issue of Proc Natl Acad Sci U S A, with permission.