Developing Drosophila embryo. Alexa Fluor 488 goat anti–mouse IgG antibody conjugate, Alexa Fluor 488 goat anti–rabbit IgG antibody conjugate and Hoechst 33342.
Formation of the cephalic furrow in the anterior end of a developing Drosophila melanogaster embryo visualized with the help of several fluorescent stains. A primary antibody to neurotactin was visualized using a red-fluorescent Cy3 dye secondary antibody (Amersham Pharmacia Biotech Ltd.). Primary antibodies to plasma membrane–bound myosin and to nuclear-localized even-skipped (Eve) protein were visualized with green-fluorescent Alexa Fluor 488 goat anti–mouse IgG antibody (Cat. No. A11001) and Alexa Fluor 488 goat anti–rabbit IgG antibody (Cat. No. A11008), respectively. The nuclei were stained with blue-fluorescent Hoechst 33342 (Cat. No. H1399, H3570, H21492). The sample was prepared by Eric Wieschaus, and the imaging was performed by Joe Goodhouse of Princeton University.