Cultured human pre-adipocytes. JC-1.
Cultured human pre-adipocytes loaded with the ratiometric mitochondrial potential indicator JC-1 (T3168) at 5 µM for 30 minutes at 37°C. In live cells, JC-1 exists either as a green-fluorescent monomer at depolarized membrane potentials or as an orange-fluorescent J-aggregate at hyperpolarized membrane potentials. Cells were then treated with 50 nM FCCP, a protonophore, to depolarize the mitochondrial membrane. Approximately 10 minutes after the addition of the uncoupler, the cells were illuminated at 488 nm and the emission was collected between 515–545 nm and 575–625 nm. Image contributed by Bob Terry, BioImage A/S, Denmark.