NIH 3T3 fibroblasts. JC-1.
NIH 3T3 fibroblasts stained with JC-1 (T3168), showing the progressive loss of red J-aggregate fluorescence and cytoplasmic diffusion of green monomer fluorescence following exposure to hydrogen peroxide. Images show the same field of cells viewed before H2O2 treatment and 5, 10 and 20 minutes after treatment. The images were contributed by Ildo Nicoletti, Perugia University Medical School.