Madin-Darby canine kidney (MDCK) cells.
Viable Madin-Darby canine kidney (MDCK) cells sequentially stained with BODIPY® FL C5-ceramide (Cat. No. D3521, B22650), LysoTracker® Red DND-99 (Cat. No. L7528) and Hoechst 33258 (Cat. No. H1398, H3569, H21491). Green-fluorescent BODIPY® FL C5-ceramide localized to the Golgi apparatus, red-fluorescent LysoTracker® Red stain accumulated in the lysosomes, and the blue-fluorescent Hoechst 33258 dye stained the nuclei. The multiple-exposure image was acquired with bandpass filters appropriate for fluorescein, Texas Red® dye and DAPI.