Adherent CRE BAG 2 cells. Alexa Fluor 594 hydrazide and Influx pinocytic cell-loading reagent.
Adherent CRE BAG 2 cells passively loaded with the membrane-impermeant polar tracer Alexa Fluor 594 hydrazide (A10438, A10442). The image on the left illustrates the relatively uniform, cytoplasmic labeling one can obtain with the Influx pinocytic cell-loading reagent (I14402), compared to the punctate labeling that results from pinocytic uptake in normal growth medium (right panel). Both images were acquired using a bandpass filter set appropriate for rhodamine dyes.