Microtubules of endothelial cells. Anti–a-tubulin antibody, Alexa Fluor 350 goat anti–mouse IgG antibody, Alexa Fluor 594 phalloidin and Alexa Fluor 488 WGA.
Microtubules of fixed bovine pulmonary artery endothelial cells localized with mouse monoclonal anti–a-tubulin antibody (A11126), which was subsequently visualized with Alexa Fluor 350 goat anti–mouse IgG antibody (A11045). Next, the F-actin was labeled with Alexa Fluor 594 phalloidin (A12381). Finally, the cells were incubated with Alexa Fluor 488 wheat germ agglutinin (W11261) to stain components of endosomal pathways. The superimposed and pseudocolored images were acquired sequentially using bandpass filter sets appropriate for DAPI, the Texas Red dye and fluorescein, respectively.