Human keratinocytes. Fluorescein concanavalin A and propidium iodide.
Apoptotic human keratinocytes that were fixed with paraformaldehyde and stained with fluorescein concanavalin A (Cat. no. C827). The cells were subsequently permeabilized with acetone, stained with propidium iodide (Cat. no. P1304MP, P3566, P21493) and visualized by confocal laser-scanning microscopy. This photomicrograph clearly shows that the green-fluorescent lectin staining outlines the apoptotic surface blebs, whereas the red-fluorescent propidium iodide stains the interior of the blebs. Image contributed by Livia Casciola-Rosen and Antony Rose, Johns Hopkins University. Reproduced from J Exp Med 179, 1317 (1994) by copyright permission of the Rockefeller University Press.