Micrococcus luteus and Bacillus cereus.
A mixed population of live and isopropyl alcohol–killed Micrococcus luteus and Bacillus cereus simultaneously stained with DAPI (Cat. No. D1306, D3571, D21490) and SYTOX® Green nucleic acid stain (Cat. No. S7020), the viability determination components of the ViaGram™ Red+ Bacterial Gram Stain and Viability Kit (Cat. No. V7023). Bacteria with intact cell membranes are stained exclusively with the cell-permeant DAPI nuclear stain and exhibit blue fluorescence, whereas cells with damaged membranes are stained with both fluorophores and exhibit green fluorescence. This image was acquired with a UV longpass optical filter set.