Yeast stained with LIVE/DEAD® Yeast Viability Kit.
A culture of Saccharomyces cerevisiae is incubated in medium containing the FUN® 1 viability indicator (Cat. No. F7030) and the counterstain Calcofluor White M2R, both of which are provided in our LIVE/DEAD® Yeast Viability Kit (Cat. No. L7009). Metabolically active yeast process the FUN® 1 dye, forming numerous red-fluorescent cylindrical structures within their vacuoles. Calcofluor stains the cell walls fluorescent blue, regardless of the yeast's metabolic state. The yeast were photographed in a single exposure through an Omega Optical triple bandpass filter set.