Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein.
Bovine pulmonary artery endothelial cells (BPAEC) incubated with the fixable, mitochondrion-selective MitoTracker® Red CMXRos (M7512). After staining, the cells were formaldehyde-fixed, acetone-permeabilized, treated with DNase-free RNase and counterstained using SYTOX® Green nucleic acid stain (S7020). Microtubules were labeled with a mouse monoclonal anti–ß-tubulin antibody, biotin-XX goat anti–mouse IgG antibody (B2763) and Cascade Blue® NeutrAvidin biotin-binding protein (A2663). This photograph was taken using multiple exposures through bandpass optical filters appropriate for Texas Red® dye, fluorescein and DAPI using a Nikon Labophot 2 microscope equipped with a Quadfluor epi-illumination system.