Multiplex FISH detection using FISH Tag™ RNA and TSA kits combined in a whole mount Drosophila embryo. The expression of Krupple (magenta) and rhomboid (orange) were detected using FISH Tag™ RNA Kits with Alexa Fluor® 647 dye (Cat. no. F32955) and Alexa Fluor® 555 dye (Cat. no. F32953) or the Multicolor Kit (Cat. no. F32956). The expression of dpp was performed with a fluorescein-labeled RNA probe (ChromaTide® fluorescein-12-UTP, Cat. no. C7603) and detected with TSA™ Kit #2 with Alexa Fluor® 488 tyramide (Cat. no. T20912) and anti-fluorescein/Oregon Green®, rabbit IgG fraction, horseradish peroxidase conjugate (Cat. no. A21253).