Intermediate filaments of astrocytes and ependymal cells in a mouse brain cryosection identified using mouse monoclonal anti-GFAP and visualized with Alexa Fluor® 488 goat anti–mouse IgG antibody. Intermediate filaments of astrocytes and ependymal cells in a 14 µm mouse brain cryosection were identified using mouse monoclonal anti–glial fibrillary monoclonal antibody (anti-GFAP, Cat. no. A21282) and visualized with green-fluorescent Alexa Fluor® 488 goat anti–mouse IgG antibody (Cat. no. A11029). Nuclei were stained with blue-fluorescent DAPI (Cat. no. D1306, D3571, D21490). The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/). 3-D reconstruction was performed using Imaris software (Bitplane AG, http://www.bitplane.com/).