HeLa cells labeled with SYTOX® Orange nucleic acid stain, anti–Golgin-97 antibody and detected using Alexa Fluor® 647 goat anti–mouse IgG antibody. Fixed, permeabilized HeLa cells were labeled with anti–Golgin-97 polyclonal antibody (Cat. no. A21270) and detected using Alexa Fluor® 647 goat anti–mouse IgG antibody (Cat. no. A21235). The cells were counterstained with SYTOX® Orange nucleic acid stain (Cat. no. S11368).