Immunofluorescent Staining using Mouse anti-Golgin-97 Monoclonal Antibody.
HeLa cells. Anti–human Golgin-97, mouse monoclonal CDF4, Alexa Fluor® 430 goat anti–mouse IgG, Alexa Fluor® 594 phalloidin, and SYTOX® Green nucleic acid stain. Fixed, permeabilized and RNase-treated HeLa cells were labeled with mouse monoclonal anti–human Golgin-97 monoclonal antibody (Cat. no. A21270) and visualized with the yellow-fluorescent Alexa Fluor® 430 goat anti–mouse IgG antibody (Cat. no. A11063). The F-actin was labeled with red-fluorescent Alexa Fluor® 594 phalloidin (Cat. no. A12381) and the nuclei were stained with the SYTOX® Green nucleic acid stain (Cat. no. S7020). This multiple-exposure image was acquired using a Nikon Diaphot fluorescent microscope equipped with a Quantix CCD camera.