Reduced background staining afforded by Image-iT® FX signal enhancer.
Reduced background staining afforded by Image-iT® FX signal enhancer. Mouse brain cryosections were permeabilized and antigen retrieval was carried out. The sections were then treated for 30 minutes with Image-iT® FX signal enhancer (Cat. no. I36933, left) or left untreated (right). Sections were labeled with the neural cell body selective antibody anti–Hu C/D (Cat. no. A21271) and visualized using TSA Kit #2 (Cat. no. T20912) with the HRP conjugate of goat anti–mouse IgG and Alexa Fluor® 488 tyramide. Sections were mounted using the reagents in the ProLong® Antifade Kit (Cat. no. P7481).