Enhanced resistance to photobleaching demonstrated by ProLong® Gold antifade reagent.
A 20-second time series showing enhanced resistance to photobleaching afforded by ProLong® Gold antifade reagent. Fixed bovine pulmonary artery endothelial cells were labeled with anti–α-tubulin(Cat. no. A11126) and visualized with fluorescein goat anti–mouse IgG (Cat. no. F2761). The samples were mounted in ProLong® Gold antifade reagent (Cat. no. P36930; top) or phosphate-buffered saline (bottom). Images were acquired at 5 second intervals using a 40x/1.3 NA oil immersion objective with continuous illumination from a standard 100 watt Hg-arc lamp.