Jurkat cells. ManLev tetraacetate, ARP and Alexa Fluor® 488 streptavidin.
Metabolic labeling of cell-surface glycoproteins. Jurkat cells were cultured for 24 hours in the presence of 25 µM ManLev tetraacetate (Cat. no. L20493). Following treatment, the cell surface–expressed, ketone-containing glycoproteins were reacted with a biotin-containing ketone-reactive reagent, ARP (Cat. no. A10550), and then visualized by incubation with Alexa Fluor® 488 streptavidin (Cat. no. S11223). The metabolically labeled glycoproteins were detected using a fluorescence microscope equipped with a filter set appropriate for FITC. No fluorescence was observed in control cells that were not treated with ManLev tetraacetate (data not shown).
