Staphylococcus aureus surface-labeled with green-fluorescent Alexa Fluor® 488 wheat germ agglutinin (Cat. no. W11261) and stained with SYTO® 59 red-fluorescent nucleic acid stain (Cat. no. S11341). A series of z-section images was acquired with a Deltavision wide-field optical sectioning microscope (Applied Precision, Inc.). A three-dimensional projection movie was generated from a deconvolved z-image stack.