Cell culture exosomes, pre-enriched using Total Exosome Isolation (from Cell Culture Media), were resuspended in PBS and bound via CD63-biotin to streptavidin-coated Dynabeads® (Exosome–Dynabeads® Streptavidin for Isolation/Detection) during an overnight incubation. The following day the Dynabeads®-bound exosomes were stained with IgG1-PE (control, grey peak), CD9-PE, or CD63-PE and analyzed by flow cytometry. A) FSC/SSC shows the singlet of bead/exosome complex [G1] and the aggregated bead/exosome complex [G2]. [B, C] Events gated on G1.