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HL-60 cells were fixed overnight with alcohol, washed, and then resuspended in 0.1% Triton® X-100/PBS/1% BSA before staining with FxCycle™ Violet stain for 30 minutes at room temperature. G0/G1 and G2/M phase histogram peaks are separated by the S-phase distribution. Analysis was performed at 405 nm excitation with a 450/50 bandpass filter.
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