Histogram of TF-1 erythroblast cells stained with FxCycle™ Far Red stain showing DNA content distribution.


TF-1 cells were fixed overnight with alcohol, washed, and then resuspended in 0.1% Triton® X-100/PBS/1% BSA before staining with FxCycle™ Far Red stain plus RNase A for 30 minutes at room temperature. G0/G1 and G2/M phase histogram peaks are separated by the S-phase distribution. Analysis was performed using 633 nm excitation with a 660/20 bandpass filter.

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