Jurkat (human T-cell leukemia) cells were treated with 10 µM EdU for 2 hours and detected according to the recommended staining protocol using Alexa Fluor® 488 azide. Cells were then stained with FxCycle™ Violet Stain (F10347). Data was collected and analyzed using an Attune® Acoustic Cytometer using 488 nm excitation and a 530/30 bandpass filter for detection of the EdU Alexa Fluor® 488 azide and 405 nm excitation and 450/40 bandpass filter for detection of the FxCycle™ Violet fluorescence. Cells in region 1 (dual positive cells) are in the S-phase, cells in region 2 are in G0/G1 phase, and cells in region 3 are in G2/M phase of the cell cycle.