Human iPSC staining using Mouse Anti Beta-Tubulin Monoclonal Antibody
Human iPSC Staining Human iPSCs were cultured on glass slides under feeder-free conditions in StemPro hESC Medium (A1000701). Cells were fixed and permed with Image-iT® Fixation/Permeabilization Kit (R37602). Oct4 (green) expression was visualized using anti-Oct4 primary Ab and Alexa Fluor® 488 secondary Ab (A11034). Tubulin (red) expression was visualized using anti-tubulin primary Ab (322600) and Alexa Fluor® 594 secondary Ab (A11005). Nuclei (blue) were labeled with NucBlue™ Fixed Cell Stain (R37606). Images were collected on the FLoid™ Cell Imaging Station (4471136).