Using anti-LC3B and the Click-iT® technology autophagy detected and glycoproteins labeled in HeLa cells
HeLa cells were fed 50uM ManNAz under regular growth conditions, after 24 hours the media was changed and 30uM chloroquine was added. The following day cells were fixed and permeabilized. ManNaz was detected using Click-iT® reactions with an alkyne Alexa Fluor® 488 (Green). Cells were labeled with 0.5ug/mL anti-LC3B with a goat anti rabbit Alexa Fluor® 647 secondary (red) and 1ug/mL Hoechst 33342 (Blue). Cells were imaged on a Zeiss LSM 710 confocal microscope.