Immunocytochemistry analysis of U2OS cells stained with p21 (CDKN1), Rabbit mAb (2H2L13) (Cat. No. 701151), ABfinity™ Recombinant Monoclonal Antibody, using (A) Alexa Fluor® 488 Goat Anti-Rabbit was used as secondary (green). (B) DAPI was used to stain the nucleus (blue) and (C) Alexa Fluor® 594 phalloidin was used to stain actin (red). (D) Composite image of cells showing nuclear localization of p21.