Detection of cell proliferation by BrdU incorporation.
Detection of cell proliferation by BrdU incorporation. NIH 3T3 cells were pulsed with BrdU (B23151) for 30 minutes before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (A21300) that was prelabeled with the Zenon Alexa Fluor 488 Mouse IgG1 Labeling Kit (Z25002). The sample was mounted with the reagents in the Prolong Antifade Kit (P7481).