Figure 2: Live/Dead® cell viability assay showing live cells stained with Calcein-AM (green) and dead cells with EthD-1 (red):
The survival of GIBCO® primary rat hippocampal and cortical neurons maintained in HIBERNATE® -E medium at ambient conditions was equivalent to the survival of neurons in NEUROBASAL® medium in a standard 5% CO2 incubator. Corresponding cultures of neurons maintained in NEUROBASAL® medium at ambient CO2 conditions showed dramatic loss of viability at 48 hours.
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