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Monitoring synaptically evoked calcium transients by two-photon laser-scanning microscopy in intact brain tissue using fluorescent dyes.

HeLa cells were transduced with Organelle Lights™ Mito-GFP (C36210) and imaged the following day in MEM media (11095) with a 40X objective.

U-2 OS cells were transduced with Cellular Lights™ Actin-RFP and Organelle Lights™ Plasma Membrane-GFP and imaged the next day. Cells were imaged in McCoys media.

HeLa cells were transduced with Organelle Lights(TM) Endosome-GFP (Cat. No. O10104).

U-2 OS cells were transduced with Cellular Lights™ Actin-RFP (Cat. No. C10127) and Cellular Lights™ MAP4-GFP (Cat. No. C10105) and imaged the next day.

U-2 OS cells were transduced with Cellular Lights™ Histone 2B-RFP (C10129) and Cellular Lights™ MAP4-GFP (C10105) and imaged the next day.

U-2 OS cells were transduced with Cellular Lights™ Actin-RFP (C10127) and Cellular Lights™ Tubilin-GFP (C10106) and imaged the next day.

MMM cells were plated in glass bottomed 35mm dishes and pHrodo™ E. coli BioParticles® conjugate for phagocytosis (P35361) were added at 50 mg/mL in HBSS (14025, supplemented with 20 mM HEPES, pH adjusted to 7.5 with NaOH) and imaged using TRITC filters.

Peptide inhibition by Western blot of (U) unstimulated and (A-H) EGF-stimulated A431 cell lysates with (A) no peptide, (B) unphosphorylated EGFr (Tyr845) peptide, (C) phospho-EGFr (Tyr845) peptide, (D) phospho-EGFr (Tyr992) peptide, (E) phospho-EGFr (Tyr1068) peptide, (F) phospho-EGFr (Tyr1086) peptide, (G) phospho-EGFr (Tyr1148) peptide, and (H) phospho-EGFr (Tyr1173) peptide using our Rb anti-phospho-EGFr(Tyr845) (Cat. No. 40-8750).

Western blot analysis of PC-3 cell lysates using our Rb anti-RUNX2 (Cat. No. 42-1000).