Technical Resources - Media Formulations
12340 - Medium 199, HEPES
Catalog Number(s):
12340030
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 50 | 0.667 |
| L-Alanine | 89 | 25 | 0.281 |
| L-Arginine hydrochloride | 211 | 70 | 0.332 |
| L-Aspartic acid | 133 | 30 | 0.226 |
| L-Cysteine hydrochloride-H2O | 176 | 0.1 | 0.000568 |
| L-Cystine 2HCl | 240 | 26 | 0.108 |
| L-Glutamic Acid | 147 | 75 | 0.51 |
| L-Glutamine | 146 | 100 | 0.685 |
| L-Histidine hydrochloride-H2O | 210 | 21.88 | 0.104 |
| L-Hydroxyproline | 131 | 10 | 0.0763 |
| L-Isoleucine | 131 | 40 | 0.305 |
| L-Leucine | 131 | 60 | 0.458 |
| L-Lysine hydrochloride | 183 | 70 | 0.383 |
| L-Methionine | 149 | 15 | 0.101 |
| L-Phenylalanine | 165 | 25 | 0.152 |
| L-Proline | 115 | 40 | 0.348 |
| L-Serine | 105 | 25 | 0.238 |
| L-Threonine | 119 | 30 | 0.252 |
| L-Tryptophan | 204 | 10 | 0.049 |
| L-Tyrosine disodium salt dihydrate | 261 | 58 | 0.222 |
| L-Valine | 117 | 25 | 0.214 |
| Vitamins | |||
| Alpha-tocopherol Phosphate | 702 | 0.01 | 0.0000142 |
| Ascorbic Acid | 176 | 0.05 | 0.000284 |
| Biotin | 244 | 0.01 | 0.000041 |
| Choline chloride | 140 | 0.5 | 0.00357 |
| D-Calcium pantothenate | 477 | 0.01 | 0.000021 |
| Folic Acid | 441 | 0.01 | 0.0000227 |
| Menadione (Vitamin K3) | 172 | 0.01 | 0.0000581 |
| Niacinamide | 122 | 0.025 | 0.000205 |
| Nicotinic acid (Niacin) | 123 | 0.025 | 0.000203 |
| Para-Aminobenzoic Acid | 137 | 0.05 | 0.000365 |
| Pyridoxal hydrochloride | 204 | 0.025 | 0.000123 |
| Pyridoxine hydrochloride | 206 | 0.025 | 0.000121 |
| Riboflavin | 376 | 0.01 | 0.0000266 |
| Thiamine hydrochloride | 337 | 0.01 | 0.0000297 |
| Vitamin A (acetate) | 328 | 0.1 | 0.000305 |
| Vitamin D2 (Calciferol) | 397 | 0.1 | 0.000252 |
| i-Inositol | 180 | 0.05 | 0.000278 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 200 | 1.8 |
| Ferric nitrate (Fe(NO3)-9H2O) | 404 | 0.7 | 0.00173 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120 | 97.67 | 0.814 |
| Potassium Chloride (KCl) | 75 | 400 | 5.33 |
| Sodium Bicarbonate (NaHCO3) | 84 | 2200 | 26.19 |
| Sodium Chloride (NaCl) | 58 | 6100 | 105.17 |
| Sodium Phosphate monobasic (NaH2PO4) anhydrous | 138 | 140 | 1.01 |
| Other Components | |||
| 2-deoxy-D-ribose | 134 | 0.5 | 0.00373 |
| Adenine sulfate | 404 | 10 | 0.0248 |
| Adenosine 5'-phosphate | 347 | 0.2 | 0.000576 |
| Adenosine 5'-triphosphate | 605 | 1 | 0.00165 |
| Cholesterol | 387 | 0.2 | 0.000517 |
| D-Glucose (Dextrose) | 180 | 1000 | 5.56 |
| Glutathione (reduced) | 307 | 0.05 | 0.000163 |
| Guanine hydrochloride | 188 | 0.3 | 0.0016 |
| HEPES | 238 | 5960 | 25.04 |
| Hypoxanthine Na | 136 | 0.4 | 0.00294 |
| Phenol Red | 376.4 | 20 | 0.0531 |
| Ribose | 150 | 0.5 | 0.00333 |
| Sodium Acetate | 82 | 50 | 0.61 |
| Thymine | 126 | 0.3 | 0.00238 |
| Tween 80® | 20 | ∞ | |
| Uracil | 112 | 0.3 | 0.00268 |
| Xanthine-Na | 152 | 0.34 | 0.00224 |
Reference:
- Morgan, J.F. and Campbell, M.E. (1955) J. Natl. Cancer Inst., 16:557.
- Morgan, J.F., Morton, H.J. and Parker R.C. (1950) Proc. Soc. Exp. 73:1.
