Technical Resources - Media Formulations

12330 - McCoy's 5A (modified), HEPES

Catalog Number(s):
12330031

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 7.5 0.1
L-Alanine 89 13.9 0.156
L-Arginine hydrochloride 211 42.1 0.2
L-Asparagine 132 45 0.341
L-Aspartic acid 133 19.97 0.15
L-Cysteine 121 31.5 0.26
L-Glutamic Acid 147 22.1 0.15
L-Glutamine 146 219.2 1.5
L-Histidine hydrochloride-H2O 210 20.96 0.0998
L-Hydroxyproline 131 19.7 0.15
L-Isoleucine 131 39.36 0.3
L-Leucine 131 39.36 0.3
L-Lysine hydrochloride 183 36.5 0.199
L-Methionine 149 14.9 0.1
L-Phenylalanine 165 16.5 0.1
L-Proline 115 17.3 0.15
L-Serine 105 26.3 0.25
L-Threonine 119 17.9 0.15
L-Tryptophan 204 3.1 0.0152
L-Tyrosine 181 18.1 0.1
L-Valine 117 17.6 0.15
Vitamins
Ascorbic Acid 176 0.5 0.00284
Biotin 244 0.2 0.00082
Choline chloride 140 5 0.0357
D-Calcium pantothenate 477 0.2 0.000419
Folic Acid 441 10 0.0227
Niacinamide 122 0.5 0.0041
Nicotinic acid (Niacin) 123 0.5 0.00407
Para-Aminobenzoic Acid 137 1 0.0073
Pyridoxal hydrochloride 204 0.5 0.00245
Pyridoxine hydrochloride 206 0.5 0.00243
Riboflavin 376 0.2 0.000532
Thiamine hydrochloride 337 0.2 0.000593
Vitamin B12 1355 2 0.00148
i-Inositol 180 36 0.2
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 100 0.901
Magnesium Sulfate (MgSO4-7H2O) 246 200 0.813
Potassium Chloride (KCl) 75 400 5.33
Sodium Bicarbonate (NaHCO3) 84 2200 26.19
Sodium Chloride (NaCl) 58 5100 87.93
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 580 4.2
Other Components
Bacto-Peptone 600 ∞
D-Glucose (Dextrose) 180 3000 16.67
Glutathione (reduced) 307 0.5 0.00163
HEPES 238 5958 25.03
Phenol Red 376.4 10 0.0266

Reference:

  1. Hsu, T.C. and Kellogg, D.S., Jr. (1960) J. Nat. Cancer Inst., 25:221.
  2. Iwakata, S. and Grace, J.T., Jr. (1964) N.Y.J. Med., 64:2279.
  3. McCoy, T.A., Maxwell, M. and Kruse, P.F. (1959) Proc. Soc. Exper. Biol. Med., 100:115. 
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