Technical Resources - Media Formulations

12200 - IMDM, powder

Catalog Number(s):
12200036 ,12200069

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 30 0.4
L-Alanine 89 25 0.281
L-Arginine hydrochloride 211 84 0.398
L-Asparagine (freebase) 150 25 0.167
L-Aspartic acid 133 30 0.226
L-Cystine 2HCl 240 91.4 0.381
L-Glutamic Acid 147 75 0.51
L-Glutamine 146 584 4
L-Histidine hydrochloride-H2O 210 42 0.2
L-Isoleucine 131 105 0.802
L-Leucine 131 105 0.802
L-Lysine hydrochloride 183 146 0.798
L-Methionine 149 30 0.201
L-Phenylalanine 165 66 0.4
L-Proline 115 40 0.348
L-Serine 105 42 0.4
L-Threonine 119 95 0.798
L-Tryptophan 204 16 0.0784
L-Tyrosine disodium salt 225 104 0.462
L-Valine 117 94 0.803
Vitamins
Biotin 244 0.013 0.0000533
Choline chloride 140 4 0.0286
D-Calcium pantothenate 477 4 0.00839
Folic Acid 441 4 0.00907
Niacinamide 122 4 0.0328
Pyridoxal hydrochloride 204 4 0.0196
Riboflavin 376 0.4 0.00106
Thiamine hydrochloride 337 4 0.0119
Vitamin B12 1355 0.013 0.0000096
i-Inositol 180 7.2 0.04
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 165 1.49
Magnesium Sulfate (MgSO4) (anhyd.) 120 97.67 0.814
Potassium Chloride (KCl) 75 330 4.4
Potassium Nitrate (KNO3) 101 0.076 0.000752
Sodium Chloride (NaCl) 58 4500 77.59
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 125 0.906
Sodium Selenite (Na2SeO3-5H20) 263 0.0173 0.0000658
Other Components
D-Glucose (Dextrose) 180 4500 25
HEPES 238 5958 25.03
Phenol Red 376.4 15 0.0399
Sodium Pyruvate 110 110 1

Reference:

  1. Dulbecco, R. and Freeman, G. (1959) Virology 8:396. 
  2. Iscove, N.N. and Melchers, F. (1978) J. Exper. Med., 147:923.
  3. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) Virology 12:185.
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