Technical Resources - Media Formulations

CMRL Medium-1066 (1X) liquid

Contains no L-glutamine

CMRL media are well suited for the growth of Earle's "L" cells. They are also especially useful for cloning monkey kidney cell cultures and for growth of other mammalian cell lines when enriched with horse or calf serum. CMRL 1066 is particularly rich in nucleosides and some vitamins.

Catalog Number(s): 11530037

COMPONENTS	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
Glycine	75	50	0.667
Hydroxy L-proline	131	10	0.0763
L-Alanine	89	25	0.281
L-Arginine hydrochloride	211	70	0.332
L-Aspartic acid	133	30	0.226
L-Cysteine hydrochloride-H2O	176	260	1.48
L-Cystine	240	26	0.108
L-Glutamic Acid	147	75	0.51
L-Histidine hydrochloride-H2O	210	20	0.0952
L-Isoleucine	131	20	0.153
L-Leucine	131	60	0.458
L-Lysine hydrochloride	183	70	0.383
L-Methionine	149	15	0.101
L-Phenylalanine	165	25	0.152
L-Proline	115	40	0.348
L-Serine	105	25	0.238
L-Threonine	119	30	0.252
L-Tryptophan	204	10	0.049
L-Tyrosine disodium salt dihydrate	225	58	0.258
L-Valine	117	25	0.214
Vitamins
Ascorbic Acid	176	50	0.284
Biotin	244	0.01	0.000041
Cholesterol	387	0.2	0.000517
Choline chloride	140	0.5	0.00357
D-Calcium pantothenate	477	0.01	0.000021
Folic Acid	441	0.01	0.0000227
Niacinamide	122	0.025	0.000205
Nicotinic acid (Niacin)	123	0.025	0.000203
Para-Aminobenzoic Acid	137	0.05	0.000365
Pyridoxal hydrochloride	204	0.025	0.000123
Pyridoxine hydrochloride	206	0.025	0.000121
Riboflavin	376	0.01	0.0000266
Thiamine hydrochloride	337	0.01	0.0000297
i-Inositol	180	0.05	0.000278
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	111	200	1.8
Magnesium Sulfate (MgSO4) (anhyd.)	120	97.7	0.814
Potassium Chloride (KCl)	75	400	5.33
Sodium Bicarbonate (NaHCO3)	84	2200	26.19
Sodium Chloride (NaCl)	58	6800	117.24
Sodium Phosphate monobasic (NaH2PO4-H2O)	138	140	1.01
Other Components
2'Deoxyadenosine	251	10	0.0398
2'Deoxycytidine	227	10	0.0441
2'Deoxyguanosine	267	10	0.0375
5-Methyl-deoxycytidine	225	0.1	0.000444
Co-carboxylase	461	1	0.00217
Coenzyme A	768	2.5	0.00326
D-Glucose (Dextrose)	180	1000	5.56
Diphosphopyridine nucleotide (NAD)	663	7	0.0106
FAD (flavin adenine dinucleotide)	786	1	0.00127
Glutathione (reduced)	307	10	0.0326
Phenol Red	376.4	20	0.0531
Sodium acetate-3H2O	136	83	0.61
Sodium glucuronate-H2O	236	4.2	0.0178
Thymidine	242	10	0.0413
Triphosphopyridine Nucleotide (NADP)	743	1	0.00135
Tween 80®		5	∞
Uridine 5'- triphosphate	484	1	0.00207