Technical Resources - Media Formulations

11605 - Grace's Insect Medium, Supplemented

Catalog Number(s):
11605045 ,11605086 ,11605094 ,11605102

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 650 8.67
L-Alanine 89 225 2.53
L-Arginine hydrochloride 211 700 3.32
L-Asparagine 132 350 2.65
L-Aspartic acid 133 350 2.63
L-Cystine 2HCl 313 28.68 0.0916
L-Glutamic Acid 147 600 4.08
L-Glutamine 146 600 4.11
L-Histidine 155 2500 16.13
L-Isoleucine 131 50 0.382
L-Leucine 131 75 0.573
L-Lysine hydrochloride 183 625 3.42
L-Methionine 149 50 0.336
L-Phenylalanine 165 150 0.909
L-Proline 115 350 3.04
L-Serine 105 550 5.24
L-Threonine 119 175 1.47
L-Tryptophan 204 100 0.49
L-Tyrosine disodium salt 225 62.14 0.276
L-Valine 117 100 0.855
beta-Alanine 89 200 2.25
Vitamins
Biotin 244 0.01 0.000041
Choline chloride 140 0.2 0.00143
D-Calcium pantothenate 477 0.02 0.0000419
Folic Acid 441 0.02 0.0000454
Nicotinic acid (Niacin) 123 0.02 0.000163
Para-Aminobenzoic Acid 137 0.02 0.000146
Pyridoxine hydrochloride 206 0.02 0.0000971
Riboflavin 376 0.02 0.0000532
Thiamine hydrochloride 337 0.02 0.0000593
i-Inositol 180 0.02 0.000111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 500 4.5
Magnesium Chloride (anhydrous) 95 1070 11.26
Magnesium Sulfate (MgSO4) (anhyd.) 120 1358 11.32
Potassium Chloride (KCl) 75 2800 37.33
Sodium Bicarbonate (NaHCO3) 84 350 4.17
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 1013 7.34
Other Components
Alpha-Ketoglutaric acid 146 370 2.53
D-Fructose 180 400 2.22
D-Glucose (Dextrose) 180 700 3.89
Fumaric acid 116 55 0.474
Lactalbumin hydrolysate 3303 ∞
Malic acid 134 670 5
Succinic acid 118 60 0.508
Sucrose 342 26680 78.01
Yeastolate 3330 ∞

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