Technical Resources - Media Formulations

11710 - Glasgow's MEM

Catalog Number(s):
11710035

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
L-Arginine hydrochloride 211 42 0.199
L-Cystine 2HCl 313 31 0.099
L-Glutamine 146 292 2
L-Histidine hydrochloride-H2O 210 21 0.1
L-Isoleucine 131 52 0.397
L-Leucine 131 52 0.397
L-Lysine hydrochloride 183 73 0.399
L-Methionine 149 15 0.101
L-Phenylalanine 165 33 0.2
L-Threonine 119 47.6 0.4
L-Tryptophan 204 8 0.0392
L-Tyrosine disodium salt dihydrate 261 52 0.199
L-Valine 117 46.8 0.4
Vitamins
Choline chloride 140 2 0.0143
D-Calcium pantothenate 477 2 0.00419
Folic Acid 441 2 0.00454
Niacinamide 122 2 0.0164
Pyridoxal hydrochloride 204 2 0.0098
Riboflavin 376 0.2 0.000532
Thiamine hydrochloride 337 2 0.00593
i-Inositol 180 3.6 0.02
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 200 1.8
Ferric Nitrate (Fe(NO3)3"9H2O) 404 0.1 0.000248
Magnesium Sulfate (MgSO4) (anhyd.) 120 97.67 0.814
Potassium Chloride (KCl) 75 400 5.33
Sodium Bicarbonate (NaHCO3) 84 2750 32.74
Sodium Chloride (NaCl) 58 6400 110.34
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 124 0.899
Other Components
D-Glucose (Dextrose) 180 4500 25
Phenol Red 376.4 15 0.0399

Reference:

  1. House, W., (1964) Medical Research Council, Institute of Virology,  University of Glasgow, Scotland, (November). 
  2. MacPherson, I. and Stoker, M., (1962)  Virology 16:147.
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