Technical Resources - Media Formulations

11765 - Ham's F-12 Nutrient Mix

Catalog Number(s):
11765047 ,11765054 ,11765062 ,11765070

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 7.5 0.1
L-Alanine 89 8.9 0.1
L-Arginine hydrochloride 211 211 1
L-Asparagine-H2O 150 15.01 0.1
L-Aspartic acid 133 13.3 0.1
L-Cysteine hydrochloride-H2O 176 35.12 0.2
L-Glutamic Acid 147 14.7 0.1
L-Glutamine 146 146 1
L-Histidine hydrochloride-H2O 210 21 0.1
L-Isoleucine 131 4 0.0305
L-Leucine 131 13.1 0.1
L-Lysine hydrochloride 183 36.5 0.199
L-Methionine 149 4.5 0.0302
L-Phenylalanine 165 5 0.0303
L-Proline 115 34.5 0.3
L-Serine 105 10.5 0.1
L-Threonine 119 11.9 0.1
L-Tryptophan 204 2.04 0.01
L-Tyrosine disodium salt dihydrate 262 7.81 0.0298
L-Valine 117 11.7 0.1
Vitamins
Biotin 244 0.0073 0.0000299
Choline chloride 140 14 0.1
D-Calcium pantothenate 477 0.5 0.00105
Folic Acid 441 1.3 0.00295
Niacinamide 122 0.036 0.000295
Pyridoxine hydrochloride 206 0.06 0.000291
Riboflavin 376 0.037 0.0000984
Thiamine hydrochloride 337 0.3 0.00089
Vitamin B12 1355 1.4 0.00103
i-Inositol 180 18 0.1
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 33.22 0.299
Cupric sulfate (CuSO4-5H2O) 250 0.0025 0.00001
Ferric sulfate (FeSO4-7H2O) 278 0.834 0.003
Magnesium Chloride (anhydrous) 95 57.22 0.602
Potassium Chloride (KCl) 75 223.6 2.98
Sodium Bicarbonate (NaHCO3) 84 1176 14
Sodium Chloride (NaCl) 58 7599 131.02
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142 142 1
Zinc sulfate (ZnSO4-7H2O) 288 0.863 0.003
Other Components
D-Glucose (Dextrose) 180 1802 10.01
Hypoxanthine Na 159 4.77 0.03
Linoleic Acid 280 0.084 0.0003
Lipoic Acid 206 0.21 0.00102
Phenol Red 376.4 1.2 0.00319
Putrescine 2HCl 161 0.161 0.001
Sodium Pyruvate 110 110 1
Thymidine 242 0.7 0.00289

Reference:

  1. Ham, R. G. (1965) Proc. Nat. Acad. Sci., 53, 288.
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