Technical Resources - Media Formulations
12390 - Ham's F-10 Nutrient Mix, HEPES
Catalog Number(s):
12390035
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75 | 7.5 | 0.1 |
| L-Alanine | 89 | 9 | 0.101 |
| L-Arginine hydrochloride | 211 | 211 | 1 |
| L-Asparagine-H2O | 150 | 15 | 0.1 |
| L-Aspartic acid | 133 | 13 | 0.0977 |
| L-Cysteine | 121 | 25 | 0.207 |
| L-Glutamic Acid | 147 | 14.7 | 0.1 |
| L-Glutamine | 146 | 146 | 1 |
| L-Histidine hydrochloride-H2O | 210 | 23 | 0.11 |
| L-Isoleucine | 131 | 2.6 | 0.0198 |
| L-Leucine | 131 | 13 | 0.0992 |
| L-Lysine hydrochloride | 183 | 29 | 0.158 |
| L-Methionine | 149 | 4.5 | 0.0302 |
| L-Phenylalanine | 165 | 5 | 0.0303 |
| L-Proline | 115 | 11.5 | 0.1 |
| L-Serine | 105 | 10.5 | 0.1 |
| L-Threonine | 119 | 3.6 | 0.0303 |
| L-Tryptophan | 204 | 0.6 | 0.00294 |
| L-Tyrosine disodium salt dihydrate | 261 | 2.62 | 0.01 |
| L-Valine | 117 | 3.5 | 0.0299 |
| Vitamins | |||
| Biotin | 244 | 0.024 | 0.0000984 |
| Choline chloride | 140 | 0.7 | 0.005 |
| D-Calcium pantothenate | 477 | 0.7 | 0.00147 |
| Folic Acid | 441 | 1.3 | 0.00295 |
| Niacinamide | 122 | 0.6 | 0.00492 |
| Pyridoxine hydrochloride | 206 | 0.2 | 0.000971 |
| Riboflavin | 376 | 0.4 | 0.00106 |
| Thiamine hydrochloride | 337 | 1 | 0.00297 |
| Vitamin B12 | 1355 | 1.4 | 0.00103 |
| i-Inositol | 180 | 0.5 | 0.00278 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111 | 33.29 | 0.3 |
| Cupric sulfate (CuSO4-5H2O) | 250 | 0.0025 | 0.00001 |
| Ferric sulfate (FeSO4-7H2O) | 278 | 0.834 | 0.003 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120 | 74.62 | 0.622 |
| Potassium Chloride (KCl) | 75 | 285 | 3.8 |
| Potassium Phosphate monobasic (KH2PO4) | 136 | 83 | 0.61 |
| Sodium Bicarbonate (NaHCO3) | 84 | 1200 | 14.29 |
| Sodium Chloride (NaCl) | 58 | 6900 | 118.97 |
| Sodium Phosphate dibasic (Na2HPO4) anhydrous | 142 | 153.7 | 1.08 |
| Zinc sulfate (ZnSO4-7H2O) | 288 | 0.0288 | 0.0001 |
| Other Components | |||
| D-Glucose (Dextrose) | 180 | 1100 | 6.11 |
| HEPES | 238 | 5958 | 25.03 |
| Hypoxanthine Na | 159 | 4.7 | 0.0296 |
| Lipoic Acid | 206 | 0.2 | 0.000971 |
| Phenol Red | 376.4 | 1.2 | 0.00319 |
| Sodium Pyruvate | 110 | 110 | 1 |
| Thymidine | 242 | 0.7 | 0.00289 |
